OPTIMIZATION OF IN HOUSE POLYMERASE CHAIN REACTION FOR HIGH RISK HUMAN PAPILLOMAVIRUS (HPV 18) E1 GENE-BASED DETECTION

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UEU » Journal » Bioteknologi
Posted by [email protected] at 11/11/2021 12:54:50 • 531 Views

OPTIMIZATION OF IN HOUSE POLYMERASE CHAIN REACTION FOR HIGH RISK HUMAN PAPILLOMAVIRUS (HPV 18) E1 GENE-BASED DETECTION

Created by :
Aroem Naroeni ( 0329057204 )
Lifda Nirmala Putri Arrasul (20160308005) ; Henny Saraswati ; Asep Deni Iskandar

Subject:	VIRUS KANKER SERVIKS HPV
Alt. Subject :	VIRUS CERVICAL CANCER HPV
Keyword:	Human Papillomavirus Polymerase Chain Reaction E1 gene

Description:

Human Papillomavirus is a virus that can infect human and a causative agent for cervical cancer. This disease mostly caused by HPV type 16 and 18. Polymerase Chain Reaction (PCR) is one of the molecular biology method can be used to detect HPV in sample. Gen E1 in HPV genome has a role in virus replication and transcription, and relatively conserved. This gene can be used in HPV genotyping and detection. This research aim is to find the PCR optimal reaction for HPV type 18 detection. The E1 gene data from the National Center for Biotechnology Information (NCBI) was used in designing primer with Primer-BLAST NCBI. Selection of the primers was done by in silico analyzes using DINAmelt and Mfold. Optimization of annealing temperature and primer concentration was done for selected primers. The result showed that optimal annealing temperature is 59oC with optimal primer concentration is 600 nM.

Date Create	:	11/11/2021
Type	:	Text
Format	:	pdf
Language	:	Indonesian
Identifier	:	UEU-Journal-11_2030
Collection ID	:	11_2030

Source :
Review of International Geographical Education 11(6), SPRING, 2021

Relation Collection:
Fakultas Ilmu-Ilmu Kesehatan

Coverage :
Civitas Akademika Universitas Esa Unggul

Rights :
@2021 Perpustakaan Universitas Esa Unggul

Publication URL :
https://digilib.esaunggul.ac.id/optimization-of-in-house-polymerase-chain-reaction-for-high-risk-human-papillomavirus-hpv-18-e1-genebased-detection-22459.html

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