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UEU » Journal » Bioteknologi Posted by [email protected] at 05/01/2021 12:58:35 • 405 Views
SEL FEEDER DAN CONDITIONED MEDIUM UNTUK KULTUR SEL PUNCA EMBRIONIK MENCIT SEBAGAI MODEL UNTUK PROPAGASI SEL PUNCA EMBRIONIKCreated by :
Aroem Naroeni ( none )
Subject: | SEL PUNCA REGENERATIF | Alt. Subject : | STEM CELL CONDITIONED MEDIUM | Keyword: | JARINGAN PENYAKIT KEDOKTERAN |
Description:
Sel punca sangat menjanjikan dalam bidang kedokteran regeneratif karena dapat menyembuhkan berbagai
penyakit karena kerusakan jaringan. Meskipun begitu, sumber sel punca yang dapat digunakan untuk
keperluan tersebut sangat terbatas. Sel darah pusat dan sumsum tulang belakang saat iniadalah merupakan
sumber sel punca yang paling banyak digunakan. Beberapa macam teknik sedang dikembangkan untuk
mengisolasi, propagasi dan differensiasi sel punca. Perkembangan induced-pluripotent stem cell (iPS)
untuk propagasi sel punca juga sedang dikembangkan. Pada penelitian ini, kami mengembangkan kultur
sel untuk propagasi sel punca dengan menggunakan sel feeders dan conditioned medium. Sel punca
diisolasi dari embrio mencit 13 hari setelah kopulasi. Setelah dikultur dalam media tersebut maka
diperoleh sekelompok sel punca yang dikelilingi oleh sel fibroblas. Sel punca dapat dikembangkan dan
dipertahankan sifat kepuncaannya dengan menggunakan sel fibroblas sebagai sel feeder dan penambahan
conditioned medium pada media kulturnya.
Alt. Description
Stem cell is very promising for regenerative medicine. Unfortunately, the sources of stem cells are
limited. Umbilical cord, bonne marrow are now the most used as stem cell source. Many techniques are
now being developed to isolate, propagate and differentiate stem cells. The development of inducedpluripotent
stem cells that reprogram adult cells to stem cells are being developed as well. In this
research we have developed stem cell culture for the propagation of stem cells by using feeder cells and
conditioned medium. Stem cells were isolated from mice embryonic 13 days after copulation. We
obtained bulks of stem cells surrounded with mouse fibroblast cells. Stem cells require this mouse
fibroblast cells as a feeder and conditioned medium, Medium that have been added with supernatant of 3
days cell culture
Date Create | : | 05/01/2021 | Type | : | Text | Format | : | pdf | Language | : | Indonesian | Identifier | : | UEU-Journal-11_0394 | Collection ID | : | 11_0394 |
Source : IJOBB Volume 1, Nomor 2, Desember 2017
Relation Collection: Civitas Akademika Universitas Esa Unggul
Coverage : Fakultas Ilmu-Ilmu Kesehatan
Rights : @2021 Perpustakaan Universitas Esa Unggul
Publication URL : https://digilib.esaunggul.ac.id/sel-feeder-dan-conditioned-medium-untuk-kultur-sel-punca-embrionik-mencit-sebagai-model-untuk-propagasi-sel-punca-embrionik-17747.html
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UEU-Journal-17747-11_0394.pdf - 896 KB
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