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UEU » Journal » Bioteknologi
Posted by [email protected] at 05/01/2021 12:58:35  •  446 Views


SEL FEEDER DAN CONDITIONED MEDIUM UNTUK KULTUR SEL PUNCA EMBRIONIK MENCIT SEBAGAI MODEL UNTUK PROPAGASI SEL PUNCA EMBRIONIK

Created by :
Aroem Naroeni ( none )



SubjectSEL PUNCA
REGENERATIF
Alt. Subject STEM CELL
CONDITIONED MEDIUM
KeywordJARINGAN
PENYAKIT
KEDOKTERAN

Description:

Sel punca sangat menjanjikan dalam bidang kedokteran regeneratif karena dapat menyembuhkan berbagai penyakit karena kerusakan jaringan. Meskipun begitu, sumber sel punca yang dapat digunakan untuk keperluan tersebut sangat terbatas. Sel darah pusat dan sumsum tulang belakang saat iniadalah merupakan sumber sel punca yang paling banyak digunakan. Beberapa macam teknik sedang dikembangkan untuk mengisolasi, propagasi dan differensiasi sel punca. Perkembangan induced-pluripotent stem cell (iPS) untuk propagasi sel punca juga sedang dikembangkan. Pada penelitian ini, kami mengembangkan kultur sel untuk propagasi sel punca dengan menggunakan sel feeders dan conditioned medium. Sel punca diisolasi dari embrio mencit 13 hari setelah kopulasi. Setelah dikultur dalam media tersebut maka diperoleh sekelompok sel punca yang dikelilingi oleh sel fibroblas. Sel punca dapat dikembangkan dan dipertahankan sifat kepuncaannya dengan menggunakan sel fibroblas sebagai sel feeder dan penambahan conditioned medium pada media kulturnya.


Alt. Description

Stem cell is very promising for regenerative medicine. Unfortunately, the sources of stem cells are limited. Umbilical cord, bonne marrow are now the most used as stem cell source. Many techniques are now being developed to isolate, propagate and differentiate stem cells. The development of inducedpluripotent stem cells that reprogram adult cells to stem cells are being developed as well. In this research we have developed stem cell culture for the propagation of stem cells by using feeder cells and conditioned medium. Stem cells were isolated from mice embryonic 13 days after copulation. We obtained bulks of stem cells surrounded with mouse fibroblast cells. Stem cells require this mouse fibroblast cells as a feeder and conditioned medium, Medium that have been added with supernatant of 3 days cell culture

Date Create:05/01/2021
Type:Text
Format:pdf
Language:Indonesian
Identifier:UEU-Journal-11_0394
Collection ID:11_0394


Source :
IJOBB Volume 1, Nomor 2, Desember 2017

Relation Collection:
Civitas Akademika Universitas Esa Unggul

Coverage :
Fakultas Ilmu-Ilmu Kesehatan

Rights :
@2021 Perpustakaan Universitas Esa Unggul


Publication URL :
https://digilib.esaunggul.ac.id/sel-feeder-dan-conditioned-medium-untuk-kultur-sel-punca-embrionik-mencit-sebagai-model-untuk-propagasi-sel-punca-embrionik-17747.html




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