IN-SILICO ANALYSIS FOR CRYI GENE AMPLIFICATION FROM BACILLUS THURINGIENSIS

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UEU » Journal » Bioteknologi
Posted by [email protected] at 19/11/2020 15:04:04 • 669 Views

IN-SILICO ANALYSIS FOR CRYI GENE AMPLIFICATION FROM BACILLUS THURINGIENSIS

Created by :
Febriana Dwi Wahyuni ( None )
Henny Saraswati Kartika Sari Dewi

Subject:	BIOTEKNOLOGI
Alt. Subject :	BIOTECHNOLOGY
Keyword:	BIOINFORMATICS CRY GENE PCR PRIMER IN SILICO ANALYSIS BACILLUS THURINGIENSIS

Description:

Bacillus thuringiensis is one type of bacteria that has been used as a microbiological control agent for pests and a vector of plant disease. The presence of Cry proteins inside the B. thuringiensis can be acted as a specific insect repellent that only toxic to certain insects. The CryI protein is toxic to Lepidoptera insects, which can attack various types of plants. Polymerase Chain Reaction (PCR) is a standard method that can be used to amplify the gene encoding CryI proteins from B. thuringiensis. This research aimed to design a primer candidate for cryI gene amplification from B. thuringiensis. In silico analysis for designing cryI primer was carried out using some software, such as BLAST for searching cryI gene sequence, Bioedit for sequences alignment, and DINAmelt for analyzing dimer structure of the primers. Ten primer candidates were successfully obtained based on the result of the primer3 software. A pair of primer was selected to amplify the cryI gene, with a forward primer 5�- CGGTGAATGCCCTGTTTACT -3� and reverse primer 5�CGGTCTGGTTGCCTATTGAT -3�. Amplification of the cryI gene by PCR method using selected primer resulting in a PCR product with a length of approximately 200 bp

Date Create	:	19/11/2020
Type	:	Text
Format	:	PDF
Language	:	Indonesian
Identifier	:	UEU-Journal-11_0591
Collection ID	:	11_0591

Source :
Bioedukasi Vol.XVIII No.1 April 2020

Relation Collection:
Fakultas Ilmu Kesehatan

Coverage :
Civitas Akademika Universitas Esa Unggul

Rights :
@2020 Perpustakaan Universitas Esa Unggul

Publication URL :
https://digilib.esaunggul.ac.id/insilico-analysis-for-cryi-gene-amplification-from-bacillus-thuringiensis-17334.html

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