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UEU » Journal » Bioteknologi Posted by [email protected] at 19/11/2020 15:04:04 • 706 Views
IN-SILICO ANALYSIS FOR CRYI GENE AMPLIFICATION FROM BACILLUS THURINGIENSISCreated by :
Febriana Dwi Wahyuni ( None ) Henny Saraswati Kartika Sari Dewi
Subject: | BIOTEKNOLOGI | Alt. Subject : | BIOTECHNOLOGY | Keyword: | BIOINFORMATICS CRY GENE PCR PRIMER IN SILICO ANALYSIS BACILLUS THURINGIENSIS |
Description:
Bacillus thuringiensis is one type of bacteria that has been used as a microbiological control agent
for pests and a vector of plant disease. The presence of Cry proteins inside the B. thuringiensis can
be acted as a specific insect repellent that only toxic to certain insects. The CryI protein is toxic to
Lepidoptera insects, which can attack various types of plants. Polymerase Chain Reaction (PCR) is a
standard method that can be used to amplify the gene encoding CryI proteins from B. thuringiensis.
This research aimed to design a primer candidate for cryI gene amplification from B.
thuringiensis. In silico analysis for designing cryI primer was carried out using some software, such
as BLAST for searching cryI gene sequence, Bioedit for sequences alignment, and DINAmelt for
analyzing dimer structure of the primers. Ten primer candidates were successfully obtained based on
the result of the primer3 software. A pair of primer was selected to amplify the cryI gene, with a
forward primer 5�- CGGTGAATGCCCTGTTTACT -3� and reverse primer 5�CGGTCTGGTTGCCTATTGAT
-3�. Amplification of the cryI gene by PCR method using selected
primer resulting in a PCR product with a length of approximately 200 bp
Date Create | : | 19/11/2020 | Type | : | Text | Format | : | PDF | Language | : | Indonesian | Identifier | : | UEU-Journal-11_0591 | Collection ID | : | 11_0591 |
Source : Bioedukasi Vol.XVIII No.1 April 2020
Relation Collection: Fakultas Ilmu Kesehatan
Coverage : Civitas Akademika Universitas Esa Unggul
Rights : @2020 Perpustakaan Universitas Esa Unggul
Publication URL : https://digilib.esaunggul.ac.id/insilico-analysis-for-cryi-gene-amplification-from-bacillus-thuringiensis-17334.html
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