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UEU » Undergraduate Theses » Bioteknologi
Posted by [email protected] at 15/10/2020 13:56:04  •  626 Views


OPTIMASI REAKSI IN HOUSE POLYMERASE CHAIN REACTION UNTUK DETEKSI HIGH RISK HUMAN PAPILLOMAVIRUS (HPV 18) BERBASIS GEN E1

Created by :
LIFDA NIRMALA PUTRI ARRASULI ( 20160308005 )



SubjectPAPILLOMAVIRUS MANUSIA
REAKSI RANTAI POLIMERASE
GEN E1
Alt. Subject HUMAN PAPILLOMAVIRUS
POLYMERAGE CHAIN REACTIONS
GEN E1
KeywordHuman Papillomavirus
Polymerase Chain Reaction
gen E1.

Description:

Human Papillomavirus merupakan virus yang dapat menginfeksi manusia dan dapat menyebabkan kanker serviks. Umumnya, kanker serviks disebabkan oleh HPV tipe 16 dan 18. Polymerase Chain Reaction (PCR) merupakan salah satu metode biologi molekuler yang digunakan untuk deteksi HPV pada sampel. Gen E1 pada genom HPV berperan dalam proses replikasi dan transkripsi virus dan umumnya lestari untuk setiap genotipe. Gen E1 juga dapat digunakan dalam penentuan genotipe HPV dan untuk deteksi infeksi HPV. Penelitian ini bertujuan untuk mencari reaksi PCR yang optimal dalam deteksi HPV tipe 18. Desain primer dibuat berdasarkan data dari National Center for Biotechnology Information (NCBI) dan dibuat dengan Primer-BLAST NCBI menggunakan gen E1. Primer diseleksi dengan analisis in silico menggunakan DINAmelt dan Mfold. Optimasi terhadap suhu annealing dan konsentrasi primer dilakukan menggunakan primer yang telah diseleksi. Diperoleh suhu annealing yang paling optimal adalah 59oC dengan konsentrasi primer 600 nM

Contributor:
  1. Aroem Naroeni, DEA, Ph.D
Date Create:15/10/2020
Type:Text
Format:PDF
Language:Indonesian
Identifier:UEU-Undergraduate-20160308005-
Collection ID:20160308005-


Source :
Undergraduate Theses of biotechnology

Relation Collection:
Fakultas Ilmu-Ilmu Kesehatan

Coverage :
Civitas Akademika Universitas Esa Unggul

Rights :
@2020 Perpustakaan Universitas Esa Unggul


Publication URL :
https://digilib.esaunggul.ac.id/optimasi-reaksi-in-house-polymerase-chain-reaction-untuk-deteksi-high-risk-human-papillomavirus-hpv-18-berbasis-gen-e1-17048.html




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Chain , E1. , Human , Human Papillomavirus , Papillomavirus , Polymerase , Polymerase Chain Reaction , Reaction , gen , gen E1.



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