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UEU » Scientific Articles » Bioteknologi Posted by [email protected] at 18/11/2020 13:45:47 • 503 Views
IN SILICO ANALYSIS FOR DETECTION OF CRYII GENE FROM LOCAL ISOLATES OF
BACILLUS THURINGIENSISCreated by :
Seprianto ( None ) Febriana Dwi Wahyuni Henny Saraswati
Subject: | BIOKTEKNOLOGI | Alt. Subject : | BIOTECHNOLOGY | Keyword: | BACILLUS THURINGIENSIS IN SILICO LOCAL ISOLATES CRYII GENE GRADIENT PCR
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Description:
Bacillus thuringiensis (Bt) is a gram-positive bacteria that can produce crystal proteins and exhibit a high
insecticidal activity. One of the cry proteins is cryII gene that encoding an insecticidal protein against the
Lepidoptera insect, especially in the Helicoverpa armigera species, which is a corn borer. This study aimed
to obtain a suitable primer candidate to amplify and characterize cryII genes from Bacillus thuringiensis
local isolates. In silico analysis was carried out for the purpose of amplification cryII gene, using several
software such as BLAST to search sequence cryII gene, Bioedit for sequence alignment, SnapGene for
analysis of genes within the genome annotation, and Primer3Plus for online primer design via
https://primer3plus.com. A pair primer was selected to represent of cryII gene with a forward primer (cry2F)
5�- ATCTGGTCTCATAGGGGCGA-3� and reverse primer (cry2R) 5�-CGAGCTGTCGTGTTGCTTTG-3�
with GC content percentage 55%. The purity of DNA Bacillus thuringiensis isolates B327 and B432 using
nanoquant with a mark on each isolate at 1.88 and 1.99. Detection of the cryII gene from local isolates of Bt
strain B327 and B432 were successfully amplified by Polymerase Chain Reaction (PCR) gradient technique
using annealing temperatures at 50 and 54�C, with DNA band size of �500 bp.
Contributor | : |
- Radisti Ayu Praptiwi
| Date Create | : | 18/11/2020 | Type | : | Text | Format | : | PDF | Language | : | Indonesian | Identifier | : | UEU-Article-5_0560 | Collection ID | : | 5_0560 |
Source : In Proceedings of the 1st International Conference on Health (ICOH 2019), pages 127-132
Relation Collection: Fakultas Ilmu Kesehatan
Coverage : Civitas Akademika Universitas Esa Unggul
Rights : @2020 Perpustakaan Universitas Esa Unggul
Publication URL : https://digilib.esaunggul.ac.id/in-silico-analysis-for-detection-of-cryii-gene-from-local-isolates-ofbacillus-thuringiensis-17312.html
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- UEU-Article-17312-5_0560.pdf - 995 KB
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