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UEU » Undergraduate Theses » Bioteknologi Posted by [email protected] at 18/10/2023 15:36:59 • 515 Views
OPTIMASI REAKSI ONE-STEP REAL -TIME PCR (RT-PCR) TERHADAP GEN BCL-2 MANUSIACreated by :
DESSY NURUL JANNAH PATTY ( 20160308006 )
Subject: | OPTIMASI ONE-STEP REAL -TIME PCR GEN BCL-2 MANUSIA | Alt. Subject : | OPTIMIZATION ONE-STEP REAL -TIME PCR HUMAN BCL-2 GENE | Keyword: | Gen BCL-2 Mekanisme apoptosis Real-Time Polymerase Chain Reaction
Isolasi RNA |
Description:
Salah satu gen yang mengatur terjadinya apoptosis pada sel adalah gen BCL-2, yang
ekspresi proteinnya berpotensi sebagai penanda prognosis kanker. Pengukuran tingkat
transkripsi gen BCL-2 dapat dilakukan dengan One-step Real-Time PCR (RT-PCR). Metode
ini dapat menggambarkan proses transkripsi gen BCL-2 yang nantinya akan ditranslasikan
menjadi protein. Optimasi reaksi qPCR perlu dilakukan untuk mendapatkan hasil pengukuran
yang valid. Penelitian kali ini dilakukan untuk mengkarakterisasi primer gen BCL-2 sebagai
bagian optimasi reaksi RT-PCR. Sampel yang digunakan adalah RNA dari orang sehat,
diisolasi dengan reagen tripure (Gene Foci, USA ). Reaksi RT-PCR dilakukan menggunakan
SensiFAST SYBR No-ROX Mix (BioLine, USA) menggunakan primer yang didapatkan dari
hasil penelitian sebelumnya. Hasil penelitian menunjukkan bahwa gen BCL-2 dapat
diamplifikasi dengan nilai Ct = 22, menggunakan RNA dengan konsentrasi 382,04 ng/μl. Perlu
dilakukan optimasi lebih lanjut untuk dapat menghasilkan kondisi RT-PCR yang sensitif dan
spesifik pada gen BCL-2
Contributor | : |
- Dr. HENNY SARASWATI, S.Si, M.Biomed
| Date Create | : | 18/10/2023 | Type | : | Text | Format | : | PDF | Language | : | Indonesian | Identifier | : | UEU-Undergraduate-20160308006 | Collection ID | : | 20160308006 |
Source : Undergraduate Theses of Biotechnology
Relation Collection: Fakultas Ilmu-Ilmu Kesehatan
Coverage : Civitas Akademika Universitas Esa Unggul
Rights : @2023 Perpustakaan Universitas Esa Unggul
Publication URL : https://digilib.esaunggul.ac.id/optimasi-reaksi-onestep-real-time-pcr-rtpcr-terhadap-gen-bcl2-manusia-30855.html
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